# Changes in Oral Papilloma Virus Infections Over Six Months in People Living with HIV

## TL;DR

This study tracks oral papilloma virus infections in people with HIV over six months, showing most infections clear, with differences between cancer-causing and non-cancer-causing strains.

## Contribution

The study provides new insights into the natural clearance and behavior of oncogenic versus non-oncogenic oral PVs in a low-resource HIV population.

## Key findings

- 60% of participants had no detectable PVs at six months, with an 84.47% clearance rate.
- Oncogenic PVs were less likely to be new infections and more likely to be cleared than non-oncogenic PVs.
- Persistence rates of oncogenic and non-oncogenic PVs did not significantly differ.

## Abstract

There is a paucity of data on changes in oral papilloma virus (PV) infection in people living with HIV (PLHIV) especially in low resource settings. The objective of this study was to determine the changes in oral PV infections in PLHIV from a low resource setting over a six-month follow-up period. This was a cohort study in which data was derived from a sub-sample of a parent study that examined oral human papilloma viruses, microbiota, and cancer in PLWHIV. This as a six-month follow up and a 2 mls saliva sample was collected from 541 participants on both visits. The saliva sample was used for DNA extraction, PV screening and typing using PCR methods. The DNA was subjected to Nanopore PV sequencing and subsequently analyzed using the phyloseq object, followed by a series of comparisons using the Phyloseq and Vegan packages in R to generate the alpha and beta diversity indices of the sequencing data from the sampled participants PV OTUs at the two visits. We found that 60% of participants had no detectable PVs at six-month follow-up, with a significant clearance rate of 84.47%. Oncogenic PVs were less likely to be detected as new infections compared to non-oncogenic PVs (Rate Ratio (RR) 0.42, 95% CI 0.31 to 0.56, P < 0.01). Oncogenic PV types were more likely cleared than non-oncogenic strains (RR 1.16, 95% CI 1.03 to 1.31, P = 0.02), but persistence rates did not significantly differ. This study highlights important trends in the natural course of oral PV infections, demonstrating that while most infections clear over time, there are distinct differences in the behavior of oncogenic versus non-oncogenic strains. These findings have important implications for the understanding of PV epidemiology and may guide future preventive and therapeutic strategies, particularly in the context of Human PV-related cancer prevention.