# Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing

## TL;DR

This study shows that cold brewing cascara at different temperatures affects its flavor, antioxidants, and mineral content, with higher temperatures increasing caffeine and copper.

## Contribution

The study is the first to show how brewing temperature affects the functional properties of cold-brewed cascara.

## Key findings

- Cold brewing increases phenolic compounds, flavonoids, and caffeine compared to hot brewing.
- Caffeine and copper concentrations are highest at 20 °C and decrease with lower brewing temperatures.
- Minerals and phenolics mainly differentiate hot-brewed cascara, while antioxidants and acidity separate cold-brewed samples.

## Abstract

Cascara, the dried husk of coffee cherries, has attracted attention as a potential beverage due to its unique flavour profile and potential health benefits. Traditionally, cascara is prepared using hot brewing methods. However, recent interest in cold brewing methods has led to research on how temperature affects the functional properties of cascara beverages.

Colour (CIE L*a*b*), total dissolved solids and titratable acidity were determined in cascara beverages prepared at 5, 10, 15 and 20 °C. The concentration of phenols and flavonoids, as well as antioxidant properties were evaluated using spectrophotometric methods. Caffeine, chlorogenic acid and melanoidins were quantified by HPLC. The mineral composition was determined using inductively coupled plasma mass spectrometry (ICP-MS). The results were compared with a hot-brewed cascara beverage.

Cold brewing resulted in significantly higher concentrations of total phenolic compounds, expressed as gallic acid equivalents (ranging from 309 to 354 mg/L), total flavonoids, expressed as quercetin equivalents (11.8–13.6 mg/L), and caffeine (123–136 mg/L) than the hot-brewed cascara beverage sample (p<0.05). Temperature had a noticeable effect on most variables, although the effect appeared to be random. In particular, concentrations of caffeine (p<0.01) and copper (p<0.001) were highest in beverages prepared at 20 °C and decreased with decreasing brewing temperature. Multivariate analysis showed that minerals (As, Co, Mn, Sn, Mg and Ca), hue and phenolic concentration contributed to the first principal component, which mainly differentiated the hot-brewed sample. Antioxidant-related variables, total titratable acidity and Se contributed most to the second principal component, which facilitated the separation of samples brewed at 5 °C.

To our knowledge, this is the first study to suggest that temperature affects the functional properties of cascara beverage produced by the cold brewing method. Experimental evidence supports the existence of a direct proportionality between caffeine and copper concentrations and brewing temperature.