MOSMAP: Mosquito metagenome analysis pipeline

TL;DR

MosMAP is a user-friendly pipeline for analyzing mosquito metagenomes, streamlining complex bioinformatics tasks.

Contribution

MosMAP integrates multiple tools into a simplified pipeline for mosquito metagenome analysis.

Findings

MosMAP showed high concordance with standard workflows in read retention and taxonomic accuracy.

The pipeline supports microbiology, ecology, and vector-borne disease research by simplifying metagenomic analysis.

Abstract

MosMAP is a bioinformatics pipeline designed for mosquito metagenome analysis. MosMAP automates essential processes like quality
control, taxonomic classification, species abundance estimation and visualization by integrating tools such as Trimgalore, Kraken 2,
Bracken and Krona into a user-friendly workflow. Each of these tools is integrated to ensure a smooth and efficient workflow from raw
data to interpretable results. The pipeline simplifies complex bioinformatics tasks, making them accessible to researchers with limited
computational expertise. MosMAP demonstrated high concordance with standard bioinformatics workflows such as Kraken and Bracken in terms
of read retention, taxonomic accuracy and abundance estimation when applied to metagenomes of mosquito collected in Bhopal, India. This
accessible pipeline promotes the simplification of meta-genomics, supporting research in microbiology, ecology and vector-borne
diseases.