# The effects of benzimidazole and electrical stimulation on peripheral nerve regeneration after short- and long-term injury

## TL;DR

This study examines how benzimidazole and electrical stimulation affect nerve recovery after different injury durations in rats.

## Contribution

The study introduces a novel combination of benzimidazole and electrical stimulation for peripheral nerve regeneration.

## Key findings

- Electrical stimulation significantly increased myelinated axon counts in short-term injury groups.
- Benzimidazole treatment alone did not significantly affect axon counts.
- Both treatments improved histological nerve structure in injured groups.

## Abstract

This research investigated the effects of benzimidazole (BZ) and electrical stimulation (ES) on peripheral nerve regeneration after short- and long-term injury and assessed functional recovery by means of stereological, histological, and electrophysiological analyses. Fifty-four male albino Wistar rats were divided into nine groups of six animals each. No treatment or surgery was applied to the control (CONT) group. The sciatic nerve was crushed for 5 s in the short-term injury (STI) and for 60 s in the long-term injury (LTI) groups. In the STI + BZ group and the LTI + BZ group, the rats received 25 mg/kg/day of BZ via oral gavage for 28 days. In the STI + ES and LTI + ES groups, a 3-V current was applied for 20 min daily for 28 days. In the STI + BZ + ES group and the LTI + BZ + ES groups, 3-V ES was applied for 20 min per day for 28 days following oral administration of BZ at 25 mg/kg/day for 28 days. All groups were subjected to electrophysiological, electron microscopic, stereological, and statistical analyses. The stereological analyses revealed a significant increases in the numbers of myelinated axons in the STI + ES groups compared with the STI (p < 0.01). BZ treatment yielded no significant differences in the numbers of myelinated axons in the groups (p > 0.05). Histological evaluation of the STI and LTI groups showed that ES and BZ treatment positively affect the histological structure of the nerve.