# Analysis of the Cytoprotective Effect of Morus alba L. Fruits as a Means of Transporting the Avulsed Teeth

## TL;DR

This study explores whether Morus alba L. fruit extracts can protect cells and serve as a transport medium for avulsed teeth.

## Contribution

The study introduces Morus alba L. fruit extracts as a novel potential transport medium for avulsed teeth.

## Key findings

- E2 extract showed highly significant cytoprotective effects compared to E1.
- Significant differences were observed between E2A and E2B, and E2B and E2C concentrations.
- Morus alba L. extract shows potential as a transport medium for avulsed teeth.

## Abstract

This study aims to test the cytoprotective effect of Morus alba L. fruit extracts on cell viability and its suitability as a transport medium for avulsed teeth.

Materials and Methods. Two Morus alba L. fruit extracts were synthesized, E1 and E2. The E1 extract was composed of fresh mulberry fruits, whereas the E2 extract was made from dehydrated fruits. The cytoprotective effect of the E1 and E2 extracts was determined using MTS testing over 5000 and 10,000 cells, after a 48 h incubation period, and sampling times of 1 h and 3 h. The concentrations tested were 50 μg/mL (E1A and E2A), 150 μg/mL (E1B and E2B), and 250 μg/mL (E1C and E2C). The data was analyzed using the one-way analysis of variance (ANOVA) test (p < 0.05) and post hoc LSD (least significant difference) analysis (p < 0.1).

Results. The post hoc LSD analysis based on the concentration of the E1 extract showed statistically differences (p < 0.1), and for the E2 extract, the results were highly significant (p = 0.011). As a function of concentration between the populations, there are significant differences between E1A and E1B (p = 0.071) and between E1B and E1C (p = 0.084), and statistically insignificant differences between E1A and E1C (p = 0.919). Significant differences were also detected between the E2A and E2B extracts (p = 0.047) and between E2B and E2C (p = 0.004).

Conclusions. Analyzing the preliminary results of our study, we can conclude that the M. alba L. extract can be considered a potential transport medium for avulsed teeth.