# Quantitative SISCOM assessment for epileptogenic zone localization: insights from a multicenter study comparing two software platforms in temporal lobe epilepsy

## TL;DR

This study compares two software tools for localizing the epileptogenic zone in temporal lobe epilepsy and finds similar accuracy but different technical outcomes.

## Contribution

Demonstrates that SPM can be a cost-effective alternative to proprietary software for SISCOM analysis in epilepsy surgery planning.

## Key findings

- Both SPM and Analyze achieved 70% concordance with the epileptogenic zone reference standard.
- SPM produced smaller, more localized clusters compared to the broader coverage of Analyze.
- Concordance between the two methods was poor, indicating methodological differences.

## Abstract

Pharmacoresistant epilepsy affects around one-third of individuals with epilepsy, requiring precise diagnosis, particularly in cases where surgical resection of the epileptogenic zone (EZ) is an option. Functional imaging techniques, such as ictal-interictal subtraction SPECT coregistered to MRI (SISCOM), have proven useful in pre-surgical evaluation by improving EZ localization accuracy. However, the widespread use of SISCOM is limited by the high costs and technical complexity of commercial software. Statistical Parametric Mapping (SPM) has been demonstrated to be a viable alternative for SISCOM analysis, displaying the potential for cost-effective EZ localization.

In this retrospective study, we evaluated patients with pharmacoresistant temporal lobe epilepsy from two reference centers of epilepsy in Brazil, who underwent ictal and interictal SPECT imaging as part of their pre-surgical evaluation, achieving favorable outcomes (Engel I or II) after surgical resection. The EZ reference standard was determined according to anatomopathological findings and good clinical outcomes. SISCOM was performed using a semi-automated approach with Statistical Parametric Mapping (SPM) and a proprietary software – Analyze. Data from each method were compared to the EZ reference standard and classified as concordant, partially concordant, or discordant.

We included 20 patients, 14 (70%) with left temporal lobe epilepsy and six (30%) with right temporal lobe epilepsy. Hippocampal sclerosis was the most common pathology (80%). Both SPM and Analyze were concordant with the EZ reference standard in 14 cases (70%), showing no difference in sensitivity between the methods. However, SPM generated smaller, more localized clusters, while Analyze produced larger clusters with broader spatial coverage. Concordance between the two methods was poor (Kappa = 0.0179), reflecting methodological differences.

This study evidences technical differences between SISCOM performed with SPM and Analyze, but with similar sensitivity (70%) for EZ localization. Further studies with larger sample sizes are required to confirm these findings. The data presented here suggest that SISCOM-SPM, due to its rapid and semi-automated workflow, may offer a practical and accessible alternative to proprietary software for epilepsy surgical planning.