# High-quality genome assembly and comparative analysis reveal extensive genomic variation in Talaromyces marneffei

## TL;DR

This study provides a high-quality genome assembly of Talaromyces marneffei and reveals significant genomic variations that could improve diagnosis and treatment.

## Contribution

The study presents a high-quality genome assembly and identifies strain-specific genomic variations, including a segmental duplication and LTR retrotransposon expansions.

## Key findings

- A high-quality genome of T. marneffei ATCC 18224 was assembled with 28.9 Mb across 11 contigs.
- A strain-specific 740-kb segmental duplication was identified, mediated by a Ty1/Copia LTR retrotransposon.
- Comparative analysis revealed large-scale rearrangements and structural variations in LTR retrotransposons.

## Abstract

Talaromyces marneffei is a dimorphic fungus that transitions from a filamentous form at 25 °C to a pathogenic yeast form at 37 °C, demonstrating pathogenicity mostly in immunocompromised individuals, such as those with human immunodeficiency virus/AIDS. Though it is one of the most severe infectious fungi in Southeast Asia, the lack of comprehensive genomic analysis has hindered advancement in strain differentiation, diagnosis and treatment. In this study, we assembled a high-quality genome of T. marneffei ATCC 18224, resulting in a 28.9 Mb genome distributed across 11 contigs, using third-generation Oxford Nanopore Technologies sequencing reads. Notably, we identified a strain-specific 740-kb segmental duplication in strain ATCC 18224, potentially mediated by inserting a Ty1/Copia long terminal repeat (LTR) retrotransposon. This segmental duplication includes various functional genes, with 75 differentially expressed during its dimorphic transition. Comparative genomic analysis revealed large-scale rearrangements in strains PM1 and 11CN-20-091, which were inconsistent with the phylogenomic trees of six T. marneffei strains and required further investigation. Additionally, we observed substantial genetic structural variations in LTR retrotransposons, particularly within the Ty1/Copia family, including two significant recent expansions in strain ATCC 18224. In summary, the identification and characterization of these extensive genomic structural variations in T. marneffei contribute to a deep understanding of its genetic diversity and will facilitate improvements in genotyping, classification and genomic surveillance.