# Efficacy of Simparica Trio® against induced infections of Ancylostoma braziliense and Ancylostoma ceylanicum in dogs

## TL;DR

Simparica Trio® was shown to be 100% effective in preventing and treating hookworm infections in dogs, including multiple stages of Ancylostoma species.

## Contribution

Demonstrated 100% efficacy of Simparica Trio® against multiple developmental stages of Ancylostoma in dogs.

## Key findings

- Simparica Trio® achieved 100% efficacy against L4, L5, and adult stages of Ancylostoma braziliense.
- The treatment was also 100% effective against the adult stage of Ancylostoma ceylanicum.
- No adverse events were observed in any of the treated dogs.

## Abstract

Hookworm infections such as Ancylostoma braziliense and A. ceylanicum pose a significant threat to pets and are implicated in causing zoonotic diseases. Despite the availability of preventatives, compliance can be lacking. Increasing pet owner options and combining endo- and ectoparasite treatments might improve this compliance. In four separate studies, we investigated the efficacy of Simparica Trio® (Zoetis Inc., Parsippany, NJ, USA) containing minimum dosages of 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel against fourth- and fifth-stage larvae (L4 and L5, respectively) and adults of A. braziliense and adults of A. ceylanicum in dogs.

Four negatively controlled, randomised and blinded laboratory studies were conducted against induced infections of A. braziliense and A. ceylanicum, with the interval between infection and treatment selected to evaluate efficacy against each targeted stage. Each treated dog received a single oral dose of Simparica Trio® at the recommended label dose. Necropsy was conducted for worm recovery on day 7 or 8 post-treatment.

No treatment-related adverse events were recorded in any of the studies. No worms were recovered from any of the Simparica Trio®-treated dogs in all four studies, thus resulting in 100% efficacy (P ≤ 0.0005) of Simparica Trio® against all stages of A. braziliense and the adult stage of A. ceylanicum in dogs.

Simparica Trio® containing sarolaner, moxidectin and pyrantel was highly effective against induced infections of L4, L5 and adult stages of A. braziliense and the adult stage of A. ceylanicum in dogs.