# Pathological and Molecular Studies of Neospora caninum Infection in Aborted Bovine Foetuses in Khorasan Razavi Province, Iran

## TL;DR

This study found that Neospora caninum infection contributes to bovine abortion in dairy cattle in Iran's Khorasan Razavi Province.

## Contribution

The study provides new evidence of N. caninum's role in bovine abortion in northeast Iran using molecular and histopathological methods.

## Key findings

- N. caninum was detected in 24.76% of aborted bovine foetuses via nested PCR.
- Brain lesions and one visible cyst confirmed N. caninum infection in foetuses.
- All cattle that aborted infected foetuses had IgG antibodies against N. caninum.

## Abstract

Neospora caninum is an obligate intracellular protozoan that is well established as a causative agent of abortion in dairy cattle worldwide.

The objective of this study was to determine the role of N. caninum infection in the abortion in dairy cattle in the Khorasan Razavi Province, Iran.

From 2022 to 2024, 105 aborted bovine foetuses were collected from dairy cattle in Khorasan Razavi province. Brain samples of aborted foetuses were tested using nested PCR and histopathological examination. In addition, blood samples were collected from dairy cattle that had aborted PCR‐positive foetuses and were analysed using enzyme‐linked immunosorbent assay (ELISA).

In the present study, N. caninum infection was detected in 24.76% (26 out of 105) of aborted bovine foetuses by nested PCR analysis. The brain tissues of 20 bovine‐aborted foetuses were only suitable for histopathological examination. Lesions of the central nervous system were severe hyperaemia, perivascular cuffing, astrogliosis, mild encephalitis and focal necrosis. One foetus exhibited a 32‐µm N. caninum cyst within the brain tissue. IgG antibodies against N. caninum were identified in all dairy cattle that aborted infected foetuses through ELISA testing. Molecular, histopathological and serological findings strongly suggest that N. caninum plays a significant role in bovine abortion in dairy cattle in Khorasan Razavi Province, northeast Iran.

Aborted bovine foetuses were collected from dairy cattle in Khorasan Razavi province. Brain samples of aborted foetuses were tested to detect N.caninum infection using nested PCR and histopathological examination. In addition, blood samples were collected from dairy cattle that had aborted PCR‐positive foetuses and were analysed using ELISA
.