# Three-Dimensional Focusing Measurement Method for Confocal Microscopy Based on Liquid Crystal Spatial Light Modulator

**Authors:** Yupeng Li, Yifan Li

PMC · DOI: 10.3390/s25082620 · Sensors (Basel, Switzerland) · 2025-04-21

## TL;DR

This paper introduces a new confocal microscopy method using a liquid crystal spatial light modulator to enable precise 3D focusing for micro-nano measurements.

## Contribution

A novel non-mechanical 3D focusing method using an LC-SLM for confocal microscopy is proposed, enabling rapid and accurate spatial position measurements.

## Key findings

- The system achieves axial measurement accuracy exceeding 1 μm within a 900 μm range.
- Lateral focusing positions can be freely adjusted without regenerating phase grayscale maps.
- The maximum resolution capability is approximately 16.667 nm.

## Abstract

Micro-nano measurement represents a critical engineering focus in the advancement of micro-nano fabrication technologies. Exploring advanced micro-nano measurement methods is a key direction for driving progress in micro-nano manufacturing. This study proposes a confocal measurement method utilizing a liquid crystal spatial light modulator (LC-SLM) to simulate a binary Fresnel lens for 3D focusing, enabling the non-mechanical measurement of spatial positions on sample surfaces. Specifically, it introduces a 3D focusing method based on LC-SLM, constructs a confocal microscopy 3D focusing system, and conducts lateral focusing experiments and axial focusing experiments. Experimental results demonstrate that the system can freely adjust lateral focusing positions. Within an axial focusing range of 900 μm, it achieves axial measurement accuracy exceeding 1 μm, with a maximum resolution capability of approximately 16.667 nm. Compared to similar confocal microscopy systems, this method allows rapid adjustment of lateral focusing positions without regenerating phase grayscale maps, achieves comparable axial measurement accuracy, and enhances measurement speed.

## Full-text entities

- **Genes:** DMD (dystrophin) [NCBI Gene 1756] {aka BMD, CMD3B, DXS142, DXS164, DXS206, DXS230}
- **Diseases:** LC-SLM (MESH:D008569), injury to (MESH:D014947)
- **Chemicals:** LC-SLM (-), helium (MESH:D006371), neon (MESH:D009356)
- **Species:** Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

11 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12030815/full.md

## References

30 references — full list in the complete paper: https://tomesphere.com/paper/PMC12030815/full.md

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Source: https://tomesphere.com/paper/PMC12030815