# Seroprevalence of Brucellosis in Haryana, India: A Study Using Rose Bengal Plate Test and Enzyme-Linked Immunosorbent Assay

## TL;DR

This study estimates the prevalence of brucellosis in bovines in Haryana, India, using two tests and finds higher rates in cattle and specific regions.

## Contribution

The study provides new seroprevalence data for brucellosis in Haryana's bovine population using RBPT and i-ELISA.

## Key findings

- The overall seroprevalence of brucellosis was 5.96% in bovines.
- Cattle had a significantly higher prevalence (7.58%) compared to buffalo (4.37%).
- Zone I had the highest seroprevalence (8.73%) compared to other agroclimatic zones.

## Abstract

Brucellosis, a contagious reproductive disease of livestock, has a significant economic impact in terms of abortions and stillbirths and has zoonotic importance. A study was conducted to estimate the seroprevalence of brucellosis in a bovine population in Haryana state, India. This study was carried out on 4325 bovine serum samples (Cattle: 2151, Buffalo: 2174) using the Rose Bengal Plate Test (RBPT) and indirect enzyme-linked immunosorbent assay (i-ELISA). The seroprevalence, estimated individually by the RBPT and i-ELISA, was found to be 6.86% (95% CI: 6.11–7.62) and 6.05% (95% CI: 5.34–6.76), respectively. In total, 258 out of 4325 (5.96%; 95% CI: 5.25–6.67) samples were found to be positive by both assays. The prevalence was found to be significantly higher in the cattle population (7.58%) as compared to buffalo (4.37%) (Chi-square = 19, p < 0.001). Seroprevalence was highest in the agroclimatic zone I (8.73%), followed by zone II (7.33%) and zone III (1.45%) (Chi-square = 76.27, p < 0.001).