RNA-Seq Insight into the Impact and Mechanisms of Methyl Donor and Glycine Betaine Osmoprotectant on Polyketide Secondary Metabolism in Monascus purpureus M1
Zheng Liu, Haijing Zhang, Furong Xue, Lidan Niu, Chenchen Qi, Wei Chen, Jie Zheng, Chengtao Wang
TL;DR
This study shows how glycine betaine boosts growth and pigment production in a fungus by acting as a methyl donor and osmoprotectant.
Contribution
The paper reveals novel gene regulatory mechanisms by which glycine betaine enhances polyketide metabolism in Monascus purpureus.
Findings
GB significantly increased red pigment and monacolin K production by 120.08% and 93.4%, respectively.
Key genes like RPS15, RPS14, and ATP9 were upregulated during later growth phases under GB treatment.
GB promotes growth and polyketide metabolism by functioning as both a methyl donor and osmoprotectant.
Abstract
Glycine betaine (GB) serves as both a methyl donor and osmoprotectant in microorganisms, facilitating growth and enhancing metabolic product yields. While the polyketide metabolites from Monascus purpureus, such as Monascus pigments (MPs) and monacolin K (MK), have been extensively studied, the effects of GB on their production and the underlying molecular mechanisms remain insufficiently explored. In this study, various concentrations of GB were added to Monascus purpureus M1 cultures, followed by RNA sequencing, RT-qPCR, differential gene expression analysis, and functional enrichment to investigate the regulatory impact of GB on polyketide metabolism. Protein–protein interaction network analysis identified key upregulated genes, including RPS15, RPS14, RPS5, NDK1, EGD2, and ATP9, particularly during the later growth phases. GB significantly upregulated genes involved in stress…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsMicrobial Metabolism and Applications · Medicinal Plants and Neuroprotection · Fungal Biology and Applications