Use of a 3D Model with Reconstructed Human Epidermis Infected with Fungi and Covered with a Bovine Nail to Simulate Onychomycosis and to Evaluate the Effects of Antifungal Agents
Francesca Giulia Urso, Salvatore Del Prete, Christelle Foucher, Martina Barberis, Francesco Carriero, Amandine Bart
TL;DR
A 3D model simulating fungal nail infections was developed to test antifungal products and evaluate their effects on fungal growth and nail structure.
Contribution
A novel 3D model using reconstructed human epidermis and bovine nails to simulate onychomycosis and assess antifungal treatments.
Findings
UDN and EXlor significantly reduced fungal presence compared to the control.
UDN preserved nail structure, while EXlor and PDermP caused damage.
The 3D model effectively differentiates antifungal product efficacy and safety.
Abstract
An in vitro 3D model using fungus colonized Reconstructed Human Epidermis (RHE) represents an effective preclinical model to simulate the pathological conditions of onychomycosis. We evaluated the suitability of this 3D onychomycosis model and use it to assess the effects of topical products on fungus growth and nail structure. Five sets of differentiated RHE were colonized with Trichophyton rubrum and covered with bovine nails. Colonized RHE with no product application (CNA) served as the control. Four different products classified as medical devices were applied once daily on the nails: Urgo Damaged Nails (UDN), Excilor (EXlor), Poderm Purifying (PDermP), Poderm Booster (PDermB). After 5 days, T. rubrum presence was visualized by the Grott Methenamine Silver staining method and quantified as the sum signal intensity of processed acquisitions. Fungal hyphae and the nail structure were…
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Taxonomy
TopicsNail Diseases and Treatments · Plant Pathogens and Fungal Diseases · Fungal Biology and Applications