Host Developmental Stage and Vegetation Type Govern Root EcM Fungal Assembly in Temperate Forests

TL;DR

This study shows that the age of trees and their species type strongly influence how ectomycorrhizal fungi assemble in temperate forests.

Contribution

The study reveals a shift from deterministic to stochastic assembly of EcM fungi as trees mature, modulated by tree species identity.

Findings

Adult trees showed higher neutral process influence (33% variation) compared to juveniles (7% variation).

Host age had a stronger effect on beta diversity (R2 = 0.057) than host identity (R2 = 0.033).

Distinct EcM fungal taxa were associated with juvenile vs. adult and coniferous vs. broadleaf hosts.

Abstract

Ectomycorrhizal (EcM) fungi are critical mediators of forest succession, yet the relative contributions of stochastic (neutral) and deterministic (niche-based) processes in shaping their communities are still poorly understood. We investigated the assembly processes in root EcM fungal communities across juvenile and adult coniferous (Abies nephrolepis, Picea jezoensis, and Pinus koraiensis) and broadleaf (Acer mono, Betula platyphylla, and Quercus mongolica) tree species in northeastern China. Employing neutral theory modeling, alpha and beta diversity metrics, and a random forest analysis, we identified patterns of EcM fungal community assembly and the specific taxa associated with developmental stages of various hosts. Neutral processes contributed to the variation in fungal communities, with adult trees showing a higher explanation power (more than 33% of variation) compared to juvenile trees (less than 7% of variation), reflecting a successional shift in assembly mechanisms. Dispersal dynamics was pronounced in juveniles but diminished with host age. Additionally, alpha diversity increased with host age and was slightly moderated by host identity, while beta diversity reflected stronger effects of host age (PERMANOVA R2 = 0.057) than host identity (R2 = 0.033). Host age and identity further structured communities, with distinct taxa varying between juvenile vs. adult, and coniferous vs. broadleaf hosts. Our results demonstrate that host maturity drives a transition from deterministic to stochastic assembly, modulated by tree species identity, improving our understanding of plant–fungal dynamics during forest succession.

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