Genome-Wide Identification and Expression Analysis of m6A Methyltransferase Family in Przewalskia tangutica Maxim

TL;DR

This study identifies and analyzes m6A methyltransferase genes in Przewalskia tangutica, revealing their structure, function, and potential roles in plant development and stress response.

Contribution

The first comprehensive analysis of the MT-A70 family in Przewalskia tangutica, including gene structure, expression, and regulatory elements.

Findings

Seven PtMT genes were identified and classified into three phylogenetic groups.

PtMT genes contain cis-acting elements linked to hormones, light, and stress responses.

Expression profiles validated by RNA-seq and qPCR suggest tissue-specific regulation.

Abstract

N6-methyladenosine (m6A) RNA modification plays important regulatory roles in plant development and adaptation to the environment. However, there has been no research regarding m6A RNA methyltransferases (MT-A70) in Przewalskia tangutica Maxim. Here, we performed a comprehensive analysis of the MT-A70 family in Przewalskia tangutica (PtMTs), including gene structures, phylogenetic relationships, conserved motifs, gene location, promoter analysis, GO enrichment analysis, and expression profiles. We identified seven PtMT genes. Phylogeny analysis indicated that the seven PtMT genes could be divided into three groups; two MTA genes, three MTB genes, and two MTC genes, and domains and motifs exhibited similar patterns within the same group. These PtMT genes were found to contain a large number of cis-acting elements associated with plant hormones, light response, and stress response, suggesting their widespread regulatory function. Furthermore, the expression profiling of different tissues was investigated using RNA-seq data, and the expression of seven genes was further validated by qPCR analysis. These results provided valuable information to further elucidate the function of m6A regulatory genes and their epigenetic regulatory mechanisms in Przewalskia tangutica.

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