# miR-27a rs895819 Polymorphism and Recurrent Pregnancy Loss in Caucasian Women: A Novel Genetic Risk Factor in a Challenging Fertility Dilemma

## TL;DR

This study finds that a specific genetic variant in miR-27a increases the risk of recurrent pregnancy loss in Caucasian women.

## Contribution

The study identifies miR-27a rs895819 GG genotype as a novel genetic risk factor for recurrent pregnancy loss in Caucasian women.

## Key findings

- The GG genotype of miR-27a rs895819 is significantly associated with increased risk of recurrent pregnancy loss.
- The AA genotype is significantly associated with a lower risk of recurrent pregnancy loss.
- The AG genotype is more common in RPL patients but not statistically significant.

## Abstract

Background: This case–control study investigates whether miR-27a rs895819 A>G polymorphism is associated with an increased risk of recurrent pregnancy loss (RPL) in Caucasian Greek women. Methods: This study included 93 women with at least two unexplained miscarriages before the 24th week of gestation (RPL group) and 107 women with no pregnancy loss history (control group). The miR-27a rs895819 A>G polymorphism was detected using PCR amplification, followed by DraIII-HF restriction enzyme digestion. Results: The GG genotype was linked to a significantly higher risk of RPL (p-value = 0.00005), whereas the AA genotype was associated with a significantly lower risk (p-value = 0.00036). The AG genotype appeared more frequently in women with RPL (49.5% vs. 44.9% in controls), but the difference was not statistically significant (p-value = 0.5139). Conclusions: To our knowledge, this is the first study demonstrating that the miR-27a A>G polymorphism was significantly associated with a higher risk of recurrent miscarriage in Caucasian women. These findings provide evidence that the GG genotype may serve as a potential genetic marker for identifying women at higher risk of recurrent miscarriage, offering valuable insights for genetic counseling and reproductive medicine.