# Metabolomics Insights into Salivary Profile in Dogs with Babesia canis Infection

## TL;DR

This study explores changes in the saliva of dogs infected with Babesia canis, revealing metabolic shifts that could aid in diagnosing and treating the disease.

## Contribution

The study is the first to use salivary metabolomics to investigate host-pathogen interactions in canine babesiosis.

## Key findings

- Salivary metabolomic profiling identified 868 significantly altered features in infected dogs.
- Disruptions in amino acid, nucleotide, lipid metabolism, and energy production were observed.
- Seven specific metabolites were identified as significantly changed through targeted analysis.

## Abstract

Babesiosis is a significant vector-borne zoonotic disease with major global economic and health implications, affecting various vertebrate hosts. Babesia parasites are auxotrophic for essential nutrients, relying on their hosts for metabolic support. This study investigated salivary metabolomic changes in dogs naturally infected with Babesia canis (N = 14) compared to healthy controls (N = 14) using untargeted and targeted mass spectrometry-based approaches. Saliva, a biofluid rich in metabolites, undergoes alterations in response to systemic diseases, making it a promising medium for studying host–pathogen interactions. Metabolomic profiling was performed using a Dionex UltiMate 3000 UHPLC system coupled with a Thermo Orbitrap Q Exactive mass spectrometer. An untargeted analysis detected 2257 salivary features, of which, 868 were significantly altered, with seven metabolites identified by reference to standards. A targeted analysis revealed significant changes in seven metabolites. Functional bioinformatics indicated disruptions in amino acid, nucleotide, and lipid metabolism, alongside alterations in energy production pathways, and purine metabolism. These findings provide critical insights into the metabolic shifts underlying canine babesiosis, supporting the development of advanced diagnostic and therapeutic strategies in the future. This study highlights the intricate interplay between host and pathogen, particularly in nutrient acquisition and metabolic regulation.