# Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway

## TL;DR

This study explores how bta-let-7d influences oxidative stress in cow endometrial cells, potentially offering new ways to improve dairy cow fertility.

## Contribution

The study reveals a novel mechanism involving bta-let-7d and the IGF1R/PI3K/AKT pathway in oxidative stress response in bovine endometrial cells.

## Key findings

- Bta-let-7d suppresses the PI3K/AKT pathway, worsening oxidative damage in bovine endometrial cells.
- Oxidative stress from KMnO4 increases ROS and MDA while reducing antioxidant enzyme activities.
- Downregulation of anti-apoptotic genes and upregulation of pro-apoptotic markers occur with oxidative stress.

## Abstract

Oxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates the oxidative stress responses induced by potassium permanganate (KMnO4) in bovine endometrial epithelial cells (BEECs). Using KMnO4 to induce oxidative stress, we observed significant increases in reactive oxygen species (ROS) and malondialdehyde (MDA) levels, accompanied by decreased activities of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD). Quantitative PCR and Western blot analyses indicated a negative correlation between IGF1R and bta-let-7d expression in oxidative-stress-affected tissues, suggesting opposing roles in managing stress responses. Following KMnO4 treatment, there was marked downregulation of anti-apoptotic genes and an upregulation of pro-apoptotic markers, alongside diminished antioxidant capacity. Mechanistically, bta-let-7d targets IGF1R, leading to the suppression of the PI3K/AKT signaling pathway and exacerbating oxidative damage. In vivo experiments further confirmed the impact of KMnO4 exposure on IGF1R expression. These findings provide novel insights into the mechanisms by which KMnO4 induces oxidative stress and apoptosis in bovine uterus. They highlight the potential for therapeutic strategies targeting the bta-let-7d/IGF1R axis to enhance reproductive health management in dairy cows, offering a promising avenue for mitigating oxidative-stress-related reproductive disorders.