# SAP30 deacetylates the Tas protein to inhibit PFV replication

## TL;DR

This study shows how the SAP30 protein inhibits PFV virus replication by deacetylating the Tas protein, reducing its ability to activate viral transcription.

## Contribution

The novel finding is that SAP30 interacts with and deacetylates the Tas protein to suppress PFV replication.

## Key findings

- PFV infection increases SAP30 mRNA and protein levels via enhanced SAP30 promoter activity by the Tas protein.
- SAP30 deacetylates the Tas protein, inhibiting its transactivation of PFV promoters and replication.
- The C-terminal Sin3 interaction domain of SAP30 is critical for suppressing PFV transcription.

## Abstract

Foamy viruses (FVs), a unique class of retroviruses, establish lifelong latent infections in the host without causing symptoms, contributing to the relatively slow progress in FV research. However, key mutations in FVs can result in severe consequences due to their broad cellular tropism, underscoring the importance of studying latent FV infections.

To identify new host proteins involved in the replication of prototype foamy virus (PFV), we previously infected the human fibrosarcoma cell line HT1080 with PFV and performed transcriptomic sequencing. The analysis revealed a significant upregulation of SAP30 mRNA levels following PFV infection. Further experiments demonstrated that PFV infection enhances SAP30 promoter activity via the Tas protein, leading to increased SAP30 mRNA and protein expression. Overexpression of SAP30 inhibited PFV replication, whereas knockdown of endogenous SAP30 enhanced PFV replication. Furthermore, SAP30 interacted with the Tas protein to induce its deacetylation, thereby suppressing Tas-mediated transactivation of the PFV LTR and IP promoters. The Sin3 interaction domain at the C-terminus of SAP30 was identified as the critical domain for inhibiting PFV transcription.

Our findings suggest that SAP30 inhibits PFV replication by deacetylating the Tas protein, thereby disrupting its transcriptional activation function. Key words: prototype foamy virus; SAP30; Tas; transcription; deacetylation.