Genomic insights into the taxonomic status and bioactive gene cluster profiling of Bacillus velezensis RVMD2 isolated from desert rock varnish in Ma’an, Jordan
Sulaiman M. Alnaimat, Saqr Abushattal, Saif M. Dmour, Wajdy J. Al-Awaida, Amani M. Ayyash, Khang Wen Goh, Kamal Ahmad Qureshi, Kamal Ahmad Qureshi, Kamal Ahmad Qureshi, Kamal Ahmad Qureshi
TL;DR
A new strain of Bacillus velezensis from a desert in Jordan was studied for its genome and potential to produce useful bioactive compounds.
Contribution
The study provides genomic insights and identifies novel biosynthetic gene clusters in a desert-isolated Bacillus strain.
Findings
The genome of Bacillus velezensis RVMD2 contains 12 biosynthetic gene clusters for secondary metabolites.
Several gene clusters do not match known sequences, suggesting novel antimicrobial compounds.
The strain shows potential for bioremediation and bioactive metabolite discovery.
Abstract
Extreme environments like arid and semi-arid deserts harbor unique microbial diversity, offering rich sources of specialized microbial metabolites. This study explores Bacillus velezensis RVMD2, a strain isolated from rock varnish in the Ma’an Desert, Jordan. The genome was sequenced using the Illumina NextSeq 2000 platform, resulting in a 4,212,579 bp assembly with a GC content of 45.94%. The assembled genome comprises 112 contigs and encodes 4,250 proteins, 77 tRNA genes, and 4 rRNA genes. Phylogenetic analysis of the 16S rRNA gene indicated a 99.84% similarity to previously identified B. velezensis strains. Whole-genome phylogeny using EzBiome, MiGA, and TYGS confirmed its classification as B. velezensis. Functional annotation identified genes involved in carbohydrate metabolism, including 324 carbohydrate-active enzyme (CAZyme) genes, stress response, and secondary metabolite…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsGenomics and Phylogenetic Studies · Microbial Community Ecology and Physiology · Enzyme Production and Characterization