# Smoking and High‐Altitude Exposure Affect Intrinsic Neural Activity: A fMRI Study of Interactive Effects

## TL;DR

This study finds that both smoking and high-altitude exposure affect brain activity, with a combined impact in specific brain regions.

## Contribution

The study is among the first to investigate the interactive effects of smoking and high-altitude exposure on intrinsic brain activity using fMRI.

## Key findings

- Smoking and high-altitude exposure each independently increased ALFF in multiple brain regions.
- A significant interaction effect was observed in the right precentral gyrus between smoking and high-altitude exposure.
- Post hoc analysis showed increased ALFF in HA smokers compared to HA nonsmokers and sea-level smokers.

## Abstract

Smoking and high‐altitude (HA) exposure both adversely affect human health, with smoking linked to various cancers and high‐altitude environments causing physiological and neurological changes. Although the effects of smoking and HA exposure on brain structure and function have been studied separately, their combined impact is still rarely explored. This study aims to investigate the interactive effects of smoking and HA exposure on intrinsic brain activity using the resting‐state functional magnetic resonance imaging (rs‐fMRI) analysed by the amplitude of low‐frequency fluctuations (ALFF) method. We used a mixed sample design, including four groups: (i) HA smokers (n = 22); (ii) HA nonsmokers (n = 22); (iii) sea‐level (SL) smokers (n = 26); and (iv) SL nonsmokers (n = 26), for a total of 96 male participants. All subjects underwent resting‐state functional magnetic resonance imaging. ALFF was used to assess differences in brain activity among the four groups. Two‐way analysis of variance (ANOVA) was conducted to analyse the effects of smoking, high‐altitude exposure and their interaction on ALFF. As for the main effect of smoking, elevated ALFF was found in the right superior frontal gyrus, right middle frontal gyrus, right inferior frontal gyrus, right middle cingulate cortex and right precentral gyrus. As for the main effect of HA exposure, elevated ALFF was found in the right putamen, right insula, right inferior frontal gyrus, right middle temporal gyrus, right precentral gyrus, right inferior temporal gyrus and right fusiform. A significant interaction effect between smoking and HA exposure was observed in the right precentral gyrus. Post hoc analysis for the right precentral gyrus showed significantly increased ALFF in groups including HA versus SL smokers; HA versus SL nonsmokers; and HA smokers versus HA nonsmokers. Our findings demonstrate that both smoking and HA exposure independently influence spontaneous brain activity, with a significant interaction between the two factors in modulating brain function. These results offer a neuroimaging‐based perspective on substance addiction in high‐altitude populations and contribute to a deeper understanding of high‐altitude adaptation.

This study used ALFF analysis of rs‐fMRI to explore the effects of smoking and high‐altitude (HA) exposure on brain activity. Both factors independently increased ALFF in several brain regions, with a significant interaction in the right precentral gyrus. These findings offer neuroimaging evidence for addiction mechanisms and HA adaptation.