Direct Shoot Regeneration from the Finger Millet’s In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers

TL;DR

This study develops a reliable method to regenerate finger millet plants in the lab and confirms their genetic stability using ISSR markers.

Contribution

The paper introduces a new and efficient regeneration protocol using shoot apex and confirms genetic fidelity with ISSR markers for finger millet.

Findings

TDZ at 4.5 µM produced the highest number of shoots (17.3) in finger millet.

IBA at 2.46 µM induced 5.6 roots per shoot with an average length of 8.2 cm.

ISSR markers confirmed genetic fidelity of in vitro regenerated plantlets.

Abstract

Globally, people are cultivating finger millet, an important cereal, to improve food availability and health benefits for humans. However, the biotechnological research on this millet is limited and insufficient in this field. The primary focus of this study is to optimize an efficient regenerated protocol for initiating further plant transformation studies, using the shoot apex as an explant and various growth regulators. For example, three cytokinins (BAP, TDZ, and Kin) at different concentrations were used to induce multiple shoots of finger millet. Among these, TDZ (4.5 µM) provided the maximum number (17.3) of shoots as compared to BAP and Kin. IBA (2.46 µM), along with MS medium, was used for the induction of roots, where 5.6 roots were produced in an individual shoot and the length of the root was longer with a size of 8.2 cm after two weeks of incubation. The clonal fidelity of the in vitro regenerated plantlets of finger millet was confirmed by ISSR primers. Overall, the present work developed a robust and reliable procedure for the establishment of efficient and reproducible regeneration through the shoot apex that will be useful for the genetic improvement of this crop. The genetic enhancement of these millets as well as the successful creation of transgenic plant varieties modified for resistance to biotic and abiotic challenges in the near future would be aided by this study.

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