Study of Expression of MST3 in Myeloid Leukaemia

TL;DR

This study investigates the role of MST3 gene expression in myeloid leukaemia and finds it upregulated compared to controls.

Contribution

The study reports MST3 upregulation in myeloid leukaemia and its correlation with KRAS and NRAS genes.

Findings

MST3 expression was upregulated in myeloid leukaemia with a 2.90–8.65-fold change.

A positive correlation was found between MST3 and KRAS/NRAS gene expression.

RBC and WBC levels in ML patients differed significantly from controls.

Abstract

Myeloid leukaemia (ML) is a cancer that occurs by the accumulation of abnormally multiplied myeloid cells in bone marrow, peripheral blood, and other related tissue. MST3 is a gene of the GCK family that has a role in apoptosis, along with other cellular functions like cellular differentiation, cell cycle, metabolism, and others. Objectives: The objectives of this study were to count RBCs and WBCs, study MST3 expression in ML and control samples, and perform an in silico correlation study on the KRAS and NRAS genes. Methods: The counting of RBCs and WBCs was carried out using a hemacytometer, the expression of MST3 was studied using RT-PCR, and a correlation study was carried out using GEPIA. Results: RBC and WBC levels in ML differed from the control levels, and the expression of MST3 was found to be upregulated in ML in comparison to controls, with a 2.90–8.65-fold change, with a significant p-value > 0.05. A positive correlation in expression was also found between MST3 and KRAS and NRAS genes, with a significant r value correlation. Conclusions: From this study, it could be deduced that MST3 might have a role in ML pathogenesis, but further research is needed to study its role in the progression of the disease.

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