Profiling the expression and functional roles of mRNAs and lncRNAs associated with post-stroke aphasia

TL;DR

This study identifies specific lncRNAs and mRNAs associated with post-stroke aphasia and explores their roles in immune and inflammatory processes.

Contribution

The study provides new insights into the molecular profiles and potential mechanisms of lncRNAs and mRNAs in post-stroke aphasia.

Findings

577 DElncRNAs and 892 DEGs were identified in post-stroke aphasia patients.

DElncRNAs and DEGs were strongly enriched in immune system and inflammatory response processes.

Specific lncRNAs and mRNAs correlated with verbal behavior in PSA patients.

Abstract

Post-stroke aphasia (PSA) is one of the primary causes of post-stroke impairment, although its underlying mechanism is unknown; therefore, this study aimed to identify the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) linked to PSA and to understand the potential processes by which they may operate.

RNA sequencing was used to determine the lncRNA and mRNA expression profiles for PSA patients and healthy control peripheral blood mononuclear cells. This allowed for the discovery of lncRNAs and differentially expressed genes (DElncRNAs and DEGs). Gene Ontology (GO) and KEGG enrichment analyses were performed on these DElncRNAs and DEGs, and qPCR was used to confirm their expression. Furthermore, any correlations between these characteristics with differential expression and the language routines of PSA patients were evaluated.

In total, comparisons of the groups yielded 577 DElncRNAs and 892 DEGs. Functional enrichment analyses of these targets demonstrated the strong enrichment of co-expressed DElncRNAs and DEGs in immune system processes and the inflammatory response. The expression levels of the lncRNAs CTD-2545M3.2 and RP11-24N18.1 and the mRNAs RPS10 and LAIR2 were similarly highly connected with verbal conduct in PSA patients upon admission.

The results highlight the lncRNA and mRNA profiles linked to PSA, demonstrating the various methods via which these DElncRNAs and DEGs may influence this clinical setting.

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