Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from LDHC in lung adenocarcinoma

Ruifang Zhong; Xiaohong Guo; Chuncai Wu; Yangyi Guo; Yanli Kang; Jianbin You; Falin Chen; Qianshun Chen; Liangyuan Chen

PMC · DOI:10.3389/fimmu.2025.1564731·April 9, 2025

Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from LDHC in lung adenocarcinoma

Ruifang Zhong, Xiaohong Guo, Chuncai Wu, Yangyi Guo, Yanli Kang, Jianbin You, Falin Chen, Qianshun Chen, Liangyuan Chen

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TL;DR

This study identifies specific peptides from LDHC that can trigger immune responses in lung adenocarcinoma, suggesting their potential use in immunotherapy.

Contribution

The study identifies new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from LDHC for lung adenocarcinoma immunotherapy.

Findings

01

Three LDHC-derived peptides showed high affinity for HLA-A2 molecules.

02

P6 peptide elicited the strongest IFN-γ response and tumor cell lysis.

03

LDHC may serve as a targetable biomarker for LUAD peptide-based immunotherapy.

Abstract

Lactate dehydrogenase C (LDHC) is a kind of cancer-testis antigen (CTA) that has been reported to be a biomarker for diagnosis, efficacy evaluation, and recurrence monitoring of lung adenocarcinoma (LUAD). This study aims to assess the value of LDHC in peptide-based vaccines for LUAD immunotherapy. The LDHC recombinant protein was purified and its effect on PC9 cells was evaluated by wound healing assay, Transwell invasion, and migration assay. Ten HLA-A2-restricted LDHC-derived peptides were predicted and synthesized, and the affinity for the HLA-A2 molecule was analyzed by T2 binding assay and molecule docking. Enzyme-linked immunospot (ELISpot) and LDH cytotoxicity assay were performed to determine the interferon-γ (IFN-γ) release level and tumor cell lysis ability of peptide-induced specific cytotoxic T lymphocytes (CTLs). The LDHC recombinant protein promoted invasion and…

Linked entities

Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.

Genes2

LDHC IFNG

Proteins2

Cell lines1

PC9— Homo sapiens (Human) · Lung adenocarcinoma · Cancer cell line

Diseases4

lung adenocarcinoma cytotoxicity tumor LUAD

Figures4

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Taxonomy

TopicsImmunotherapy and Immune Responses · Cancer Immunotherapy and Biomarkers · Immune Cell Function and Interaction