# Changes in the metabolome, lipidomein, and gut microbiota in Behçet’s disease

## TL;DR

This study found that people with Behçet’s disease have distinct gut bacteria and lower levels of certain metabolites compared to healthy individuals.

## Contribution

The study reveals novel insights into gut microbiota and metabolite changes specific to Behçet’s disease.

## Key findings

- BD patients showed reduced clostridia and increased γ-proteobacteria and betaproteobacteria in gut flora.
- Several metabolites were significantly lower in BD patients, including L-phenylalanine and ascorbic acid.
- Strong associations were found between specific metabolites and microbial families like Prevotellaceae.

## Abstract

There is growing evidence that autoimmune illnesses are associated with the metabolome and microbiota. Because Behçet’s disease (BD) is not often diagnosed as a systemic disorder, the aim of this research was to investigate changes in gut flora and metabolites in BD patients.

We used 16S rRNA gut microbiota gene sequencing and UPLC-QTOF-MS analysis to gather stool and serum samples from 12 age-matched healthy controls and 17 BD patients. The correlation between changes in gut microbiota and metabolites was then further analyzed.

In contrast to healthy controls, our investigation revealed significant changes in the makeup of gut flora in BD patients. In particular, we observed that in the BD group, there was a large drop in clostridia but a noticeable rise in γ-proteobacteria and betaproteobacteria. The serum metabolomics profiles of BD patients and healthy controls may be reliably differentiated using unsupervised principal component analysis (PCA). Several metabolites, including L-phenylalaine, tricarballylic acid, beta-leucine, ketoleucine, ascorbic acid, l-glutamic acid, l-malic acid, d-glucopyranuronic acid, and methyl acetoacetate, were found to have differential expression between BD patients and healthy controls. All of these metabolites were significantly lower in the BD group. Furthermore, we discovered strong associations between the detected metabolites such as tricarballylic acid, L-malic acid, D-glucopyranuronic acid with certain microbial families, such Prevotellaceae and Alcaligenaceae.

Patients with BD were found to have significant changes in the makeup of their gut flora and metabolites.