# Effects of opium on cholesterol metabolism in rats fed normal and high-fat/high-cholesterol diet

## TL;DR

This study found that opium lowers blood cholesterol and glucose in rats but harms liver and intestinal health, especially when combined with a high-fat diet.

## Contribution

The study reveals opium's dual effects on cholesterol metabolism and tissue health in rats under normal and high-fat diets.

## Key findings

- Opium reduced serum glucose and total cholesterol levels in rats.
- Opium increased LDL-R, HMG-CoA reductase, and CYP7A1 gene expression in the liver.
- Intestinal tissue showed disorganization and destruction due to opium use.

## Abstract

There is a misconception that opium can lower blood sugar and cholesterol levels. Hence, this study aimed to investigate the influences of opium on the expression of key cholesterol metabolism genes in the liver and intestine of rats receiving a cholesterol-rich diet. Male Wistar rats were randomly divided into four groups (n = 6): normal control, opium addiction, hypercholesterolemic diet, and opium addiction received hypercholesterolemic diet. After 28 days, the blood glucose levels, liver enzymes, and cholesterol in the rat's serum were measured. The cholesterol regulatory genes and transporters such as 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, low-density lipoprotein receptor (LDL-R), cholesterol 7 alpha-hydroxylase 1 (CYP7A1) (in liver tissue), and ATP Binding cassette subfamily g member 5 and 8 (ABCG5 and ABCG8), and Niemann-Pick C1-like 1 protein (NPC1L1) (in intestinal tissue) were measured. Intestinal morphological changes were also evaluated. Opium decreased serum glucose and total cholesterol levels (P < 0.05). In contrast, the levels of liver enzymes increased compared to the normal control group (P < 0.05). Histological examinations revealed that opium caused disorganization, deformation, and destruction of cells in intestinal tissue. Real-time PCR analysis demonstrated that opium increased the expression of LDL receptor genes, HMG-CoA reductase enzyme, and CYP7A1 in the liver compared to the normal control group (P < 0.05). The changes of ABCG8 and NPC1L1 transporters in intestinal tissue were not significant. Opium had beneficial effects on blood lipid and glucose levels, but histological findings indicated destructive effects on intestinal tissues.

•High fat and high cholesterol diets had deteriorated effect of liver function in addicted animals.•High fat and high cholesterol diets had deteriorated effects on intestinal structure in addicted animals.•Opium increases the low-density lipoprotein receptor (LDL-R) gene expression.•Opium increases 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene expression.•Opium increases cholesterol 7 alpha-hydroxylase 1 (CYP7A1) reductase gene expression.

High fat and high cholesterol diets had deteriorated effect of liver function in addicted animals.

High fat and high cholesterol diets had deteriorated effects on intestinal structure in addicted animals.

Opium increases the low-density lipoprotein receptor (LDL-R) gene expression.

Opium increases 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene expression.

Opium increases cholesterol 7 alpha-hydroxylase 1 (CYP7A1) reductase gene expression.