Determining physiological responses of mussels (Mytilus edulis) to hypoxia by combining multiple sensor techniques

TL;DR

Blue mussels survive longer without oxygen when exposed to air during low tide than in hypoxic water, due to different physiological responses.

Contribution

The study combines multiple biosensors to monitor mussels' physiological responses to aerial and aqueous hypoxia in real time.

Findings

Mussels close their valves and reduce heart rate during aerial exposure, suggesting a shift to anaerobic metabolism.

Most mussels open their valves during aqueous hypoxia, increasing filtration activity to collect more oxygen.

Mussels show less interindividual variation in responses during aerial exposure compared to aqueous hypoxia.

Abstract

Intertidal bivalves survive longer without oxygen when aerially exposed during low tide than when submerged in hypoxic water. To understand this, we combined three biosensors to continuously monitor responses of individual blue mussels (Mytilus edulis) to aerial exposure in simulated low-tide conditions and during aqueous hypoxia. A valve sensor, heart rate monitor, and an in-shell oxygen microsensor simultaneously recorded behavioural and physiological responses. During aerial exposure, which often occurs in the intertidal, all individuals immediately closed their valves, rapidly depleted in-shell oxygen, and decreased their heart rate. This suggested a shift to anaerobic metabolism and reduced activity as mechanisms to save energy and survive in-shell anoxia during ‘low-tide’ conditions. At the onset of exposure to hypoxic (<1 mg O2/L) water, however, all mussels fully opened their valves, with 75% of the individuals increasing valve activity for at least 1 hour (the duration of our measurements), possibly in an attempt to collect more oxygen by increasing filtration activity. Only 25% of the mussels closed their valves after about 40 minutes of aqueous hypoxia, shifting to the energy efficient strategy used during aerial exposure. As the valves of most individuals remained open during hypoxia, a mussel does not appear to need to close its valve to begin the transition to anaerobic metabolism. Interindividual variation in responses was much lower after exposure to air compared to aqueous hypoxia when the heart rate of most mussels either steadily declined or became highly erratic. Differences in energy expenditure during these different types of exposures likely explains why most mussels, at least from the population we studied, can survive longer during exposure to air compared to aqueous hypoxia, a situation that could occur under situations of elevated temperature in waters with high nutrient loads.

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