# Inhibitory Effects of Aqueous and Hydroalcoholic Extracts from Jatobá Coat (Hymenaea courbaril L.) on Pancreatic Amylase and Starch Absorption

## TL;DR

Jatobá coat extracts can inhibit pancreatic amylase and reduce starch-induced hyperglycemia in mice.

## Contribution

The study identifies jatobá coat extracts as potential agents for glycemic control through α-amylase inhibition.

## Key findings

- Aqueous and hydroalcoholic extracts from jatobá coat inhibited α-amylase with IC50 values of 81.98 µg/mL and 51.06 µg/mL.
- Both extracts reduced starch-induced hyperglycemia in mice, with the aqueous extract showing broader dose efficacy.
- In silico analysis suggests procyanidin dimers and flavonoids may contribute to the inhibitory effects.

## Abstract

Jatobá (Hymenaea courbaril) is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá. Within this context, the present work attempts to find possible applications for the jatobá coat in glycemic control through inhibition of α-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an α-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited α-amylase. The IC50 values for the aqueous and hydroalcoholic extracts were 81.98 ± 3.53 µg/mL and 51.06 ± 0.42 µg/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the α-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá coat warrant further investigations as potential modulators of glycemia following starch ingestion.