Changes in T Lymphocytes and Cytokines After Anti-TNF Treatment in Pediatric Inflammatory Bowel Disease: Association with Response to Pharmacologic Therapy
Paula Zapata-Cobo, Sara Salvador-Martín, Sergio Gil-Manso, Marta Velasco Rodríguez-Belvís, Laura M. Palomino, Ana Moreno-Álvarez, Begoña Pérez-Moneo, Ruth García-Romero, María J. Fobelo, Diana García-Tirado, César Sánchez, Gemma Pujol-Muncunill, Oscar Segarra
TL;DR
The study examines how T lymphocytes and cytokines change after anti-TNF treatment in children with IBD, identifying potential biomarkers for treatment response.
Contribution
The study identifies specific T lymphocyte populations and cytokine changes associated with short- and long-term response to anti-TNF therapy in pediatric IBD.
Findings
Naïve Tregs are linked to primary response, while activated Tregs correlate with long-term response to anti-TNF drugs.
INF-γ levels decrease in Crohn’s disease but not in ulcerative colitis after treatment.
Memory CD8+ Tc2 cells increase in Crohn’s non-responders, and CD4+ Th17 cells in UC non-responders.
Abstract
Failure of anti-TNF therapy is a real concern in children with inflammatory bowel disease (IBD) owing to the limited therapeutic arsenal. Anti-TNF drugs modulate the immune response, a key driver of chronic inflammation in IBD. Accordingly, we analyzed changes in the frequency of T-lymphocyte and cytokine levels after 6 weeks of treatment to identify potential biomarkers of response to anti-TNF drugs. We recruited 77 patients under 18 years of age diagnosed with IBD and treated with an anti-TNF drug. Using flow cytometry and multiplex ELISA, we analyzed 31 T-lymphocyte populations and four cytokines. We identified changes in 10 populations of T lymphocytes after 6 weeks of treatment. Naïve Tregs were associated with a primary response to anti-TNF drugs, while activated Tregs were associated with long-term response. Serum INF-γ levels were decreased after anti-TNF treatment in children…
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Taxonomy
TopicsInflammatory Bowel Disease · Microscopic Colitis · Autoimmune and Inflammatory Disorders Research