# In vitro models to study viral-induced asthma exacerbation: a short review for a key issue

## TL;DR

This review discusses in vitro models for studying how viruses worsen asthma, highlighting their strengths and limitations.

## Contribution

The paper provides a focused review on in vitro models for viral-induced asthma exacerbation, emphasizing their suitability for specific aspects of the disease.

## Key findings

- Asthma involves complex interactions between multiple cell types and inflammatory pathways.
- No single in vitro model can fully represent the entire asthma physiopathology.
- Different in vitro models are useful for studying specific aspects of virus-related asthma.

## Abstract

Asthma is a heterogenous inflammatory bronchial disease involving complex mechanisms, several inflammatory pathways, and multiples cell-type networks. Bronchial inflammation associated to asthma is consecutive to multiple aggressions on epithelium, such as microbiologic, pollutant, and antigenic agents, which are responsible for both T2 and non-T2 inflammatory responses and further airway remodeling. Because asthma physiopathology involves multiple crosstalk between several cell types from different origins (epithelial, mesenchymal, and immune cells) and numerous cellular effectors, no single and/or representative in vitro model is suitable to study the overall of this disease. In this short review, we present and discuss the advantages and limitations of different in vitro models to decipher different aspects of virus-related asthma physiopathology and exacerbation.