# Modular Platform for Efficient Assembly of Multifunctional Antibodies Using Orthogonal Protein–Protein Interactions

## TL;DR

This paper introduces a new method to efficiently build multifunctional antibodies that can target multiple molecules at once, with high purity and ease of use.

## Contribution

A modular post-translational platform using orthogonal protein–protein interactions for assembling multifunctional antibodies is introduced.

## Key findings

- The platform achieved over 90% assembled scaffold and high product purity.
- Antibodies were created for cancer detection, growth inhibition, and T cell targeting.
- The method offers modularity, high efficiency, and simplified purification.

## Abstract

Multifunctional antibodies, capable of simultaneously
engaging
multiple targets, are a unique class of antibodies that have sparked
growing interest. Current approaches for making multifunctional antibodies,
including chemical conjugation or genetic modifications, suffer from
low product yield, complex structure design, and complicated manufacturing
processes. In this study, we report a modular post-translational platform
with highly specific protein–protein interactions for multifunctional
antibody assembly and an elastin-like polypeptide (ELP) for easy purification.
We generated and purified multifunctional antibodies with over 90%
assembled scaffold and overall product purity. Additionally, we assembled
antibodies with diverse applications, including detecting cancer,
inhibiting cancer cell growth, and directing T cells to cancer cells
for enhanced therapeutic efficacy. This platform offers high assembly
efficiency, easy purification, and modularity for the redesign of
antibody functions.