# Genome sequence of cluster F1 Mycobacterium smegmatis phage Fastidio

**Authors:** Matthew N. Jackson, Hyunbi Hwang, Payson C. Danielson, Ethan Walbom, Jayden Longhurst, Bartel Van Oostendorp, Christopher C. Harrell, Austin M. Johnson, Natalie A. Olsen, Parker Danielson, Thomas Wilhite, Jeffrey K. Schachterle, Staci Avery, Donald P. Breakwell, Brett E. Pickett

PMC · DOI: 10.1128/mra.00148-25 · Microbiology Resource Announcements · 2025-03-19

## TL;DR

This paper presents the genome sequence of a new bacteriophage called Fastidio that infects a specific strain of Mycobacterium smegmatis.

## Contribution

The study contributes a newly annotated genome of a Mycobacterium smegmatis phage with potential novel genes.

## Key findings

- Fastidio's genome is 55,839 base pairs long.
- The genome contains several putative novel open reading frames.
- The study enhances understanding of phage diversity in Mycobacterium smegmatis.

## Abstract

Fastidio is an F1 subcluster bacteriophage in the Caudoviricetes class, infecting Mycobacterium smegmatis strain mc²155. The genome is 55,839 base pairs in length and contains several putative novel open reading frames. The isolation, annotation, and analysis of Fastidio, together with other bacteriophages, improve our knowledge of phage diversity.

## Full-text entities

- **Species:** Mycolicibacterium smegmatis (species) [taxon 1772], Bacteriophage sp. (species) [taxon 38018]

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC11984147/full.md

## References

12 references — full list in the complete paper: https://tomesphere.com/paper/PMC11984147/full.md

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Source: https://tomesphere.com/paper/PMC11984147