# Amplicon microbiome sequencing of compost from conventional and redesigned compost buckets

**Authors:** Elizabeth Klosko, Elizabeth Hutchison, Ahmad Almomani

PMC · DOI: 10.1128/mra.00808-24 · Microbiology Resource Announcements · 2025-02-25

## TL;DR

This study compares the microbial communities in compost from two types of buckets and finds no significant difference based on bucket shape.

## Contribution

The novelty lies in using amplicon sequencing to evaluate the impact of compost bucket design on microbial communities.

## Key findings

- Proteobacteria and Firmicutes were the dominant bacterial phyla in the compost.
- Ascomycota was the primary fungal phylum identified.
- Bucket shape had no significant effect on microbial community composition.

## Abstract

Here, we report using amplicon sequencing to assess microbial growth in both conventional and pyramid-shaped compost buckets. Proteobacteria and Firmicutes were the primary bacterial phyla present, and Ascomycota the primary fungal phylum present. Bucket shape did not significantly affect microbial community composition.

## Full-text entities

- **Chemicals:** water (MESH:D014867)
- **Species:** Musa acuminata (banana, species) [taxon 4641], Bacillota (clostridial firmicutes, phylum) [taxon 1239], Solanum lycopersicum (tomato, species) [taxon 4081], Pseudomonadota (proteobacteria, phylum) [taxon 1224], Ascomycota (ascomycete fungi, phylum) [taxon 4890]

## Full text

_Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC11984133/full.md

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC11984133/full.md

## References

16 references — full list in the complete paper: https://tomesphere.com/paper/PMC11984133/full.md

---
Source: https://tomesphere.com/paper/PMC11984133