# Role of immunostimulatory deoxycytidylate-phosphate-deoxyguanylate (CpG) motifs in oral bacteria associated with oral diseases

## TL;DR

This study explores how CpG DNA motifs in oral bacteria may influence immune responses and contribute to oral diseases.

## Contribution

The study identifies CpG motif patterns in oral bacteria linked to specific diseases and their potential role in immune modulation.

## Key findings

- Sixty percent of analyzed bacteria had medium GC% content with no significant difference across disease-associated bacteria.
- A positive correlation was found between GC% content and CpG motif frequency, as well as genome size and CpG motif frequency.
- Core endodontic microbiota showed higher-than-mean frequencies of human and animal immunostimulatory motifs.

## Abstract

CpG oligodeoxynucleotide motifs in bacterial DNA with composition variations exhibit potent immunostimulation. The effect of different compositions in oral infections is unclear. This study aims to investigate CpG motifs in bacteria associated with endodontic diseases, periodontal diseases, and dental caries to elucidate their influence on host immune response.

Fifty oral bacterial genomes were selected for in silico analysis to determine GC% content and CpG motif frequency in each genome. The relationships between GC% content, CpG motif frequency, and genome size were assessed using correlation analysis. Normalisation of immunostimulatory sequences was implemented to enable unbiased comparison of frequency counts among bacteria.

Sixty percent of bacteria exhibited medium GC% content (Mdn = 44), with no significant difference among bacteria associated with these diseases (p = 0.66). A positive correlation between GC% content and CpG motif frequency, as well as genome size and CpG motifs frequency was observed. A higher-than-mean of the human immunostimulatory motif (GTCGTT, 7/14) and the mice/rabbits immunostimulatory motif (GACGTT, 9/14) was observed in core endodontic microbiota.

CpG motifs in oral bacteria might drive disease progression through host immunomodulation. Variation in bacterial CpG motifs suggests targeting these motifs offers a promising therapeutic intervention.