The effect of adipose-derived mesenchymal stem cells against high fructose diet induced liver dysfunction and dysbiosis

TL;DR

Adipose-derived stem cells help protect the liver and gut from the harmful effects of a high fructose diet by reducing oxidative stress and improving metabolic functions.

Contribution

This study demonstrates that ADMSCs can counteract high fructose diet-induced liver dysfunction and gut dysbiosis through specific molecular mechanisms.

Findings

ADMSCs improved liver function and reduced oxidative stress in rats on a high fructose diet.

ADMSCs increased adiponectin and IL10 levels while modulating IRS1 and SREBP-1 expression.

ADMSCs ameliorated gut microbiota activity and attenuated insulin resistance.

Abstract

High fructose diet (HFrD) has been approved to be involved in the pathogenesis of insulin resistance. Mesenchymal stem cells have a vital role in the treatment of various diseases including metabolic disturbances. We investigated the effect of Adipose-derived mesenchymal stem cells (ADMSCs) against HFrD-induced metabolic disorders and the molecular mechanisms for this effect. Rats were divided into 3 groups; control, HFrD, and combined HFrD with ADMSCs. We assessed liver functions, gut microbiota activity, oxidative stress, adiponectin, and IL10 levels. Also, we measured SREBP-1, IRS-1 expression using Western blot, and Malat1 expression using rt-PCR. ADMSCs antagonized metabolic abnormalities induced by HFrD in the form of improvement of liver functions and alleviation of oxidative stress. In addition, ADMSCs ameliorated gut microbiota activity besides the elevation of adiponectin and IL10 levels. ADMSCs attenuated insulin resistance through upregulation of IRS1 and downregulation of SREBP-1 and Malat1. ADMSCs can protect against HFrD-induced metabolic hazards.

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