BmMed6 modulates mating behavior by ORs and antennae structural genes in the silkworm

TL;DR

The BmMed6 gene in silkworms is essential for antenna development and influences mating behavior by regulating olfactory and structural genes.

Contribution

This study reveals BmMed6's role in modulating mating behavior through its regulation of antennae structure and olfactory receptor genes in silkworms.

Findings

BmMed6 mutants showed abnormal antennae growth, affecting mating behavior.

BmMed6 regulates genes for antennae structure and olfactory receptors.

Disruption of BmMed6 leads to downregulation of olfactory receptor genes.

Abstract

Gene expression is under strict and precise control to regulate organism development and maintain various physiological functions. The Mediator complex is a regulator of gene transcription. Our study focused on BmMed6, a component of the Mediator complex in the Bombyx mori. We construct BmMed6 mutants using the CRISPR-Cas9 system. The mutants exhibited abnormal growth patterns in their antennae, which limited their mating behavior. RNA-seq and gene expression analysis have revealed that the expression of genes associated with structural constituents of the cuticle in the antennae of the mutant was aberrant. Moreover, the deficiency of BmMed6 also caused the downregulation of olfactory receptor genes. Our findings offer novel insights into the biological role of BmMed6 in antenna growth, revealing its crucial role in regulating antenna structure and olfactory gene expression to influence mating behaviors. This discovery identifies BmMed6 as a viable new target gene for pest control.

•BmMed6 is required for the development of antenna in the silkworm•BmMed6 regulate the ORs and antennae structural genes•The abnormal antennae influence mating behaviors

BmMed6 is required for the development of antenna in the silkworm

BmMed6 regulate the ORs and antennae structural genes

The abnormal antennae influence mating behaviors

Biological sciences; Entomology; Molecular biology

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