Autocrine Wingless constricts the Drosophila embryonic gut by Ca+2-mediated repolarisation of mesoderm cells
Delia Ricolo, Francesca Tamba, Jordi Casanova
TL;DR
This study shows how Wingless (Wg) signaling in fruit fly embryos helps shape the gut by changing the structure of mesoderm cells through calcium pulses.
Contribution
The study reveals a novel mechanism by which autocrine Wg signaling induces gut constriction via Ca+2-mediated repolarization of mesodermal cells.
Findings
Wg signaling induces ClC-a transcription in mesodermal cells, leading to Ca+2 pulses.
Ca+2 pulses cause repolarization of mesodermal cells and microtubule reorganization.
Mesodermal repolarization is essential for forming the middle constriction of the Drosophila gut.
Abstract
Wg/Wnt signalling—a highly conserved transduction pathway—has most commonly been found to be involved in patterning, cell fate, or cell proliferation, but less so in shaping organs or body parts. A remarkable case of the latter is the role of Wg signalling in the midgut of the Drosophila embryo. The Drosophila embryonic midgut is divided into four chambers that arise by the formation of three constrictions at distinct sites along the midgut. In particular, Wg is responsible for the middle constriction, a role first described more than 30 years ago. However, while some partial data have been obtained regarding the formation of this gut constriction, an overall picture of the process is lacking. Here we unveil that Wg signalling leads to this constriction by inducing ClC-a transcription in a subset of mesodermal cells. ClC-a, encodes a chloride channel, which in turn prompts a Ca+2 pulse…
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Taxonomy
TopicsRNA Research and Splicing · Developmental Biology and Gene Regulation · Invertebrate Immune Response Mechanisms