# The impact of IgSF11-PKM2 pathway on gene expression in osteoclasts

## TL;DR

This study explores how the IgSF11-PKM2 pathway affects gene expression in bone-resorbing cells called osteoclasts.

## Contribution

The study identifies specific gene expression changes linked to the IgSF11-PKM2 pathway in osteoclasts.

## Key findings

- Osteoclast-related genes are altered by the absence of IgSF11.
- PKM2 activity changes also influence gene expression in osteoclasts.
- RNA sequencing reveals the impact of the IgSF11-PKM2 pathway on osteoclast gene regulation.

## Abstract

Osteoclasts are primary bone resorbing cells. Previously, we described metabolic regulation of osteoclasts through IgSF11-mediated phosphorylation of the glycolytic enzyme PKM2. Here, we report the impact of IgSF11-PKM2-mediated regulation on gene expression in osteoclasts, utilizing RNA sequencing on osteoclasts engineered to express a chimeric protein, lacking IgSF11, and pharmacologically modulating PKM2 activity. Our analysis identified osteoclast-related genes whose expression is altered by the absence of IgSF11 and by changes in PKM2 activity. This study reveals gene expression changes associated with the IgSF11-PKM2 pathway, providing new insight into its role in osteoclasts.