# Multiplex Immunofluorescent Batch Labeling of Marmoset Brain Sections

## TL;DR

This paper introduces a new method for labeling marmoset brain tissue with fluorescent dyes to study brain structure and disease.

## Contribution

The study introduces a batch-style multiplex immunohistochemistry protocol for marmoset brain sections.

## Key findings

- A set of marmoset-compatible fluorescent dyes and antibodies were characterized for labeling brain structures.
- A practical batch-style protocol was developed for high-throughput immunolabeling of multiple tissue slides.
- The method supports the creation of brain histology atlases for marmoset research.

## Abstract

The common marmoset is a small nonhuman primate that has emerged as a valuable animal model in neuroscience research. Accurate analysis of brain tissue is crucial to understand marmoset neurophysiology and to model neurodegenerative diseases. Many studies to date have complemented magnetic resonance imaging (MRI) with histochemical staining rather than immunofluorescent labeling, which can generate more informative and higher resolution images. There is a need for high‐throughput immunolabeling and imaging methodologies to generate resources for the burgeoning marmoset field, particularly brain histology atlases to display the organization of different cell types and other structures.

Here, we have characterized a set of marmoset‐compatible fluorescent dyes and antibodies that label myelin, axons, dendrites, and the iron‐storage protein ferritin, and developed a batch‐style multiplex immunohistochemistry protocol to uniformly process large numbers of tissue slides for multiple cell‐type specific markers.

We provide a practical guide for researchers interested in harnessing the potential of marmoset models to advance understanding of brain structure, function, and pathophysiology.

To support high‐throughput immunolabeling and imaging methodologies for the burgeoning marmoset field, we characterize a set of marmoset‐compatible fluorescent dyes and antibodies and provide a practical batch‐style multiplex immunohistochemistry protocol for researchers interested in harnessing the potential of marmoset models to advance understanding of brain connectomics, function, and pathophysiology.