Correction: CDC2 Mediates Progestin Initiated Endometrial Stromal Cell Proliferation: A PR Signaling to Gene Expression Independently of Its Binding to Chromatin

Abstract

Figures

C](#pone.0321979.g001) are incorrect, and that this was not observed prior to publication as the RU antagonist effect over R5020 is the same as the ICI antagonist effect over R5020. The corresponding author further clarified that the CCnd1 and β-Actin panels in [Fig 1C](#pone.0321979.g001) represent the same experimental samples run on different agarose gels, that the same cDNA template for each experimental sample was used for beta actin PCR and Ccnd1 PCR, and that the PCR products were then run on different agarose gels.

F](#pone.0321979.g002).

Full text

After publication of this article [1], concerns were raised about results presented in Fig 1. Specifically:

The corresponding author stated that lanes 5–8 of the CCnd1 and β-Actin panels in Fig 1C are incorrect, and that this was not observed prior to publication as the RU antagonist effect over R5020 is the same as the ICI antagonist effect over R5020. The corresponding author further clarified that the CCnd1 and β-Actin panels in Fig 1C represent the same experimental samples run on different agarose gels, that the same cDNA template for each experimental sample was used for beta actin PCR and Ccnd1 PCR, and that the PCR products were then run on different agarose gels.

The corresponding author also stated that the vertical discontinuities in Figs 1, 2 and 4 are due to the original experiments being carried out alongside additional treatments not included in [1]. A correct version of Fig 1 is provided here where non-adjacent bands from the same gel are indicated with solid black lines in Figs 1A, C and D, and where lanes 5–8 of the CCnd1 and β-Actin panels in Fig 1C have been updated. Updated versions of Figs 2 and 4 are provided here where non-adjacent bands from the same gel are indicated with solid black lines in Figs 2H and 4B. The corresponding author stated that they did not generate panels from lanes from different gels.

The available underlying data for Figs 1, 2H and 4B are provided here in S1 and S2 Files. The corresponding author stated that the remainder of the original raw data supporting the figures in the article are available with the exception of the raw data for the Gfi1, Pten and beta Actin gels in Fig 2F.

A member of the PLOS One Editorial Board reviewed the underlying data and updated versions of Figs 1, 2 and 4 and stated that they align with the published results and conclusions that progestin regulates endometrial stromal cell proliferation via ERK1–2 and AKT activation independent of PR binding to chromatin.

Supporting Information

S1 File The available raw image data supporting Figs 1, 2H , and 4B.This includes the underlying Ccnd1 and beta Actin agarose gels from Fig 1A, the underlying Ccnd1 (RU, ICI, PD and LY) and beta Actin (RU, ICI, PD and LY) agarose gels from Fig 1C, the underlying c-Myc, Cdkn1a, beta Actin and Ccnd1 agarose gels from Fig 1D, the underlying JunD, Usf1, Crebbp, Cyr61 and Cdkn1b agarose gels from Fig 2H, and the underlying Cdc2 and Ccnb1agarose gels from Fig 4B.(ZIP)

S2 File Underlying quantitative data for Fig 1B.(XLS)