# Selective toxicity of ascorbic acid and hydrogen peroxide on human tenon cells without harming scleral cells in vitro: A possible alternative to non-selective mitomycin C?

## TL;DR

Ascorbic acid and hydrogen peroxide may selectively kill Tenon cells in glaucoma surgery without harming other eye cells, offering a safer alternative to Mitomycin C.

## Contribution

Demonstrates selective cytotoxicity of ascorbic acid and hydrogen peroxide on Tenon fibroblasts without affecting scleral fibroblasts.

## Key findings

- Ascorbic acid (6–8 mM) and hydrogen peroxide selectively induced cell death in Tenon fibroblasts without harming scleral fibroblasts.
- Lower catalase expression in Tenon fibroblasts explains their susceptibility to ascorbic acid and hydrogen peroxide.
- Recombinant catalase neutralized the cytotoxic effects of ascorbic acid and hydrogen peroxide on Tenon fibroblasts.

## Abstract

Glaucoma, a leading cause of blindness, is often driven by elevated intraocular pressure (IOP), which damages the optic nerve. Transscleral filtration surgery reduces IOP but is frequently complicated by excessive wound healing from Tenon fibroblasts (TFs), impeding aqueous humor absorption. Mitomycin C (MMC), used for over 30 years in ophthalmic surgeries, inhibits TF proliferation but carries significant side effects, including hypotony, blebitis, and endophthalmitis, due to its non-selective cytotoxicity. MMC’s inability to entirely prevent fibrosis increases surgical failure risk, often necessitating further interventions like bleb needling. This study investigates whether ascorbic acid (AA) and hydrogen peroxide (H2O2) can selectively target TFs without damaging scleral fibroblasts (SFs) in vitro, using MMC as a benchmark.

Primary human TFs and SFs were cultured from patient trabeculectomy tissues. Cells were treated with various concentrations of MMC, AA, or H2O2. Cytotoxic effects were analyzed via live-cell imaging. Immunocytochemistry and Western Blot assessed catalase expression in both cell types and recombinant catalase was used to validate its protective effect against AA- and H2O2-induced cell death.

Short-term exposure (5 min) to 0.02%–0.04% MMC or long-term exposure to 0.00025%–0.001% MMC caused cytotoxicity in TFs and SFs, with SFs dying significantly earlier. In contrast, AA (6–8 mM) selectively induced cell death in TFs without harming SFs. H2O2 also showed selective cytotoxicity towards TFs. Lower catalase expression in TFs compared to SFs was determined via Western blot and immunocytochemistry, highlighting a mechanism for this selective effect. Recombinant catalase neutralized the cytotoxic effects of AA and H2O2 on TFs.

Unlike MMC, Ascorbic acid and hydrogen peroxide exhibit selective cytotoxicity towards Tenon fibroblasts, which may provide a safer, more targeted approach for preventing fibrosis in glaucoma surgery. Additional in vivo studies are needed to explore the clinical applicability of these findings.