# Prevalence, correlates, and health associations of 24-hour movement behaviours in Slovenian adults: a protocol for a longitudinal population-based study

**Authors:** Kaja Kastelic, Tjaša Knific, Nejc Šarabon

PMC · DOI: 10.1186/s44167-025-00073-z · 2025-03-19

## TL;DR

This study will investigate how physical activity, sedentary behavior, and sleep relate to health in Slovenian adults over time.

## Contribution

The study introduces a protocol for analyzing 24-hour movement behaviors and their longitudinal health associations in a representative adult population.

## Key findings

- The study will use accelerometers and surveys to assess movement behaviors and health outcomes.
- It will explore how socio-demographic and environmental factors correlate with movement behaviors.
- Findings will inform future guidelines for adult movement behaviors.

## Abstract

Physical activity, sedentary behaviour, and sleep (i.e., 24-hour movement behaviours) are among key determinants of health and well-being. However, epidemiological studies that investigate these behaviours while accounting for their co-dependent nature are still scarce. This article describes the protocol of a population-based study that aims to explore the prevalence and correlates of 24-hour movement behaviours among Slovenian adults and their cross-sectional and longitudinal associations with health and well-being.

Participants will be recruited through the National Health-related Lifestyle Survey study that is conducted among a national representative sample of Slovenian adults aged 18–74 years (n of invited participants every four years = 17,500). Baseline data collection will include assessment of 24-hour movement behaviours using activPAL accelerometers. Domain-specific movement behaviours, built environment, and well-being will be assessed using questionnaires. A linkage to the National Health-related Lifestyle Survey that include data on demographics (e.g., marital status, socio-economic status), health-related behaviours (e.g., diet, smoking), and health (e.g., self-reported health, comorbidities) will also be established. The follow-up data collections (every four years) will include self-reported assessments of 24-hour movement behaviours, built environment, health and well-being, and linkage to the national health-related registers.

The study will produce new knowledge on 24-hour movement behaviours, their socio-demographic and built environment correlates, and their cross-sectional and longitudinal relationship with health outcomes using compositional data analysis. It will reveal an insight into the relative importance of domain-specific and type-specific movement behaviours, informing future 24-hour movement guidelines for adults.

## Full-text entities

- **Genes:** FAS (Fas cell surface death receptor) [NCBI Gene 355] {aka ALPS1A, APO-1, APT1, CD95, FAS1, FASTM}, PTS (6-pyruvoyltetrahydropterin synthase) [NCBI Gene 5805] {aka PTPS}, SPNS1 (SPNS lysolipid transporter 1, lysophospholipid) [NCBI Gene 83985] {aka HSpin1, LAT, PP2030, SLC62A1, SLC63A1, SPIN1}
- **Diseases:** joint diseases (MESH:D007592), pain in other joints (MESH:D018771), coughing fits (MESH:D003371), cardiovascular diseases (MESH:D002318), heart attack (MESH:D009203), chronic renal failure (MESH:D007676), death (MESH:D003643), hypertension (MESH:D006973), arthritis (MESH:D001168), excessive daytime sleepiness (MESH:D006970), constipation (MESH:D003248), allergy (MESH:D004342), musculoskeletal symptoms (MESH:D009140), thyroid disease (MESH:D013959), type 2 diabetes (MESH:D003924), depression (MESH:D003866), heart failure (MESH:D006333), liver cirrhosis (MESH:D008103), angina pectoris (MESH:D000787), anxiety (MESH:D001007), bronchial asthma (MESH:D001249), swelling of (MESH:D004487), emphysema (MESH:D004646), mental illnesses (MESH:D001523), cancers (MESH:D009369), neck/shoulder pain (MESH:D020069), chronic bronchitis (MESH:D029481), diabetes (MESH:D003920), ESS (MESH:C538175), pain (MESH:D010146), daytime sleepiness (MESH:D012893), headache (MESH:D006261), gastric or duodenal ulcer (MESH:D013276), low back pain (MESH:D017116), anxiety disorders (MESH:D001008), toothache (MESH:D014098), IFIS (MESH:D012640), arthrosis (MESH:D010003), chronic fatigue (MESH:D015673), spinal diseases and disorders (MESH:D013122), legs (MESH:D010264), chest pain (MESH:D002637), COPD (MESH:D029424), Fatigue (MESH:D005221), chronic non-communicable diseases (MESH:D000073296), stroke (MESH:D020521), obesity (MESH:D009765), gestational diabetes (MESH:D016640)
- **Chemicals:** HeBEQ (-), alcohol (MESH:D000438), triglycerides (MESH:D014280), cholesterol (MESH:D002784), blood sugar (MESH:D001786)
- **Species:** Homo sapiens (human, species) [taxon 9606], Gallus gallus (bantam, species) [taxon 9031], Mus musculus (house mouse, species) [taxon 10090], Nicotiana tabacum (American tobacco, species) [taxon 4097], Meleagris gallopavo (common turkey, species) [taxon 9103]

## Figures

2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC11960283/full.md

---
Source: https://tomesphere.com/paper/PMC11960283