The development and validation of a microneutralization assay for the detection and quantification of anti-yellow fever virus antibodies in human serum
Katherine Fries, Ping Luo, Rebekah Baldwin, Raechel Goldberg, Ivan Ordonez, Lingyi Zheng, James Huleatt, Louis Devlin
TL;DR
Researchers developed a new, efficient test to detect yellow fever antibodies in blood, which could improve vaccine testing and approval processes.
Contribution
A new microneutralization assay for yellow fever virus antibodies was developed and validated for high-throughput clinical testing.
Findings
The new YF microneutralization assay showed 100% agreement with the PRNT50 at a titer of 10 in vaccinated individuals.
The assay demonstrated acceptable precision, accuracy, and specificity across various serum conditions and viruses.
The Center for Biologics Evaluation and Research confirmed the assay's suitability for vaccine licensure.
Abstract
The plaque reduction neutralization test (PRNT) has been used widely for the detection and quantitation of yellow fever (YF) virus-neutralizing antibodies in human serum; however, it is labor-intensive and challenging to adapt to high-throughput clinical testing needed for vaccine licensure. Here, we describe the development and validation of a new Vero cell-based YF microneutralization (MN) assay, with immunostaining readout, for the detection and quantification of YF virus-neutralizing antibodies in human serum. Comparison of neutralizing antibody titers measured with the YF MN assay versus the historical YF PRNT, based on a 50% reduction in plaque counts (PRNT50), demonstrated 100% serostatus agreement at a titer of 10 (1/dil) in participants with a history of YF vaccination. For validation, intra-assay precision (repeatability), intermediate precision, dilutional accuracy,…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsMosquito-borne diseases and control · Viral Infections and Vectors · Viral Infections and Outbreaks Research