# Characterization of temperature-sensitive alleles of anillin-like Mid1 and polo kinase Plo1 in Schizosaccharomyces pombe

## TL;DR

This study examines temperature-sensitive mutations in Mid1 and Plo1 proteins in fission yeast to better understand their roles in cell division.

## Contribution

The study identifies specific mutations in Mid1 and Plo1 and highlights key domains critical for cytokinesis.

## Key findings

- The Mid1 C2 domain is essential for proper cytokinesis.
- The Plo1 kinase domain's C-terminal lobe is crucial for cell division.
- Temperature-sensitive alleles reveal growth and morphological defects in mutant strains.

## Abstract

The
Schizosaccharomyces pombe
anillin-like Mid1 is important for the correct positioning of the cell division site. A key regulator of Mid1 is the polo kinase Plo1 which is important for several mitotic and cytokinetic events including spindle formation and division site placement. Here, we defined the mutations within a set of temperature-sensitive
mid1
and
plo1
alleles and compared the growth and morphological defects of the strains. This work expands the repertoire of
mid1
and
plo1
mutants for studying cytokinesis and highlights the requirement of the Mid1 C2 domain and the Plo1 kinase domain C-terminal lobe as particularly important for cytokinesis.