# Genome Sequences of Cluster E Mycobacteriophages Xandras and BigBubba **Authors:** Ashley Gledhill, Gabriel S. Gooden, Janelle Aguazul, Aanyaa Arora, Ava R. Blackledge, Michael H. Bucks, Tessa Carby, Andrew Cu, Jacob Fulkerson, Chealsea W. Gachagua, Elly Grogan, Haley H. Hanson, Gillian N. Johnston, Lola S. Norman, Lindsey M. Oak, Grant J. Oller, Het Parekh, Sakshi Patel, Sydney Putnam, Grant Spalding, Jacob Thomas, Patrick Wallace, Claire A. Rinehart, Rodney A. King PMC · DOI: 10.17912/micropub.biology.001460 · microPublication Biology · 2025-03-13 ## TL;DR This paper presents the genome sequences of two new mycobacteriophages, Xandras and BigBubba, isolated from soil and characterized for their genetic features. ## Contribution The novelty lies in the isolation and genomic characterization of two new Cluster E mycobacteriophages with CBASS antagonist proteins. ## Key findings - Xandras has a 75,179 bp genome with 144 predicted protein-coding genes and two tRNAs. - BigBubba has a 75,006 bp genome with 147 predicted protein-coding genes and two tRNAs. - Both phages contain a cyclic oligonucleotide sequestering protein (CBASS antagonist). ## Abstract Bacteriophages Xandras and BigBubba were isolated on Mycobacterium smegmatis mc 2 155 from enriched soil samples. Xandras' genome length is 75,179 bp with 144 predicted protein-coding genes and two tRNAs. BigBubba's genome length is 75,006 bp with 147 predicted protein-coding genes and two tRNAs. Each genome contains a cyclic oligonucleotide sequestering protein (CBASS antagonist). ## Full-text entities - **Chemicals:** oligonucleotide (MESH:D009841) - **Species:** Mycolicibacterium smegmatis MC2 155 (strain) [taxon 246196] ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC11953737/full.md ## Figures 1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC11953737/full.md ## References 14 references — full list in the complete paper: https://tomesphere.com/paper/PMC11953737/full.md --- Source: https://tomesphere.com/paper/PMC11953737