# Effect of novel IL-8 gene mutation on its protein structure and stability among ovarian cancer patients in Saudi Arabia

## TL;DR

A new mutation in the IL-8 gene may affect its protein structure and increase ovarian cancer risk in Saudi patients.

## Contribution

A novel non-synonymous IL-8 gene mutation (c.193G>A; p.Glu65Leu) is linked to ovarian cancer susceptibility.

## Key findings

- The p.Glu65Leu mutation alters IL-8 protein conformation and function.
- The mutation was found in five ovarian cancer patients with stage II and III tumors.
- This polymorphism may contribute to tumor susceptibility in ovarian cancer.

## Abstract

Despite advancements in therapeutics, early diagnosis of ovarian cancer (OC) remains a challenge due to its asymptomatic progression
and it is often diagnosed at an advanced stage. Interleukin-8 (IL-8), a pro-inflammatory chemokine with angiogenic activity, plays a
pivotal role in inflammation and carcinogenesis. Therefore, it is of interest to investigate the association between IL-8 polymorphisms
and OC risk. A novel non-synonymous SNP (nsSNP, c.193G>A; p.Glu65Leu) was identified in exon 2 of the IL-8 gene in 5 patients, 3 with
stage III and 2 with stage II tumor. Structural analysis suggested that the p.Glu65Leu mutation alters the IL-8 protein conformation
showing functional impairment. Thus, data shows that IL-8 c.193 G >A (Glu65Leu) polymorphism may contribute to tumor susceptibility.
However, further studies are warranted to understand the role of IL-8 polymorphism in OC.