# Determination of lamotrigine in human plasma by HPLC-PDA. Application to forensic samples

## TL;DR

This paper describes a new and validated HPLC method for measuring lamotrigine in human plasma, useful for forensic toxicology.

## Contribution

A new HPLC-PDA method for lamotrigine quantification in plasma was developed and validated for forensic use.

## Key findings

- The method is linear in the range of 0.1–10 µg/ml with a high correlation coefficient (r = 0.993).
- The method achieved a limit of detection of 0.04 µg/ml and a recovery rate of 98.9% or higher.
- The method was successfully applied to 11 postmortem blood samples in a forensic setting.

## Abstract

Therapeutic drug monitoring of plasma lamotrigine (LTG) has customarily been carried out in order to prevent some its adverse effects. For forensic purposes, determination of LTG in plasma is an useful tool in cases of accidental overdose or suicidal attempts. Currently, there are several analytical methods available including some based on LC tandem mass spectrometry techniques, but simple and accessible LC-UV methods still can be useful for the purpose. Here we report on a new high-performance liquid chromatography method for the determination of lamotrigine in human plasma which has been developed and validated including selectivity, sensitivity, accuracy, precision and recovery studies.

Lamotrigine and the internal standard chloramphenicol were extracted from plasma using liquid-liquid extraction using small volumes of buffer and ethylacetate. Detection was monitored at 305.7 and 276.0 nm for lamotrigine and chloramphenicol, respectively.

The method was linear concentration dependence within the range of 0.1–10 µg/ml, with a mean coefficient of correlation r = 0.993. The limit of detection (LOD) was 0.04 µg/ml and the limit of quantification (LOQ) was 0.1 µg/ml. Intra and interday precision values were lower than 9.0% at all concentrations studied. The intra and interday accuracy values ranged from − 7.6 to 10.1%. Recovery was found to be 98.9% or higher. The method here described was successfully applied to 11 postmortem blood samples received at the Forensic Sciences Institute of Santiago de Compostela (Spain).

A new HPLC method for the determination of lamotrigine in human plasma was developed and validated. A liquid-liquid extraction using small volumes of buffer and ethylacetate was optimized. The proposed method is suitable for forensic toxicological analysis.