# Avicennia marina endogenous promoter AMGT1P33 enhances salt tolerance in Arabidopsis by regulating exogenous salt-tolerance genes

## TL;DR

A salt-tolerant promoter from mangroves boosts salt tolerance in Arabidopsis by regulating gene expression, offering potential for crop improvement.

## Contribution

The study identifies and validates a novel promoter from Avicennia marina that enhances salt tolerance in plants.

## Key findings

- AMGT1P33 promoter drives higher GUS expression in plants compared to the 35S promoter.
- AMGT1P33-regulated AmBADH expression improves salt tolerance in yeast and Arabidopsis.

## Abstract

Mangroves form ecologically and economically important ecosystems and are a potential source of valuable genetic resources given their natural salt tolerance. However, the role that promoters play in their salt-tolerance mechanisms remains unclear.

In this study, we identified the AMGT1P33 promoter in the genome of the mangrove tree species Avicennia marina using PromPredict and then verified its promoter function according to the transient expression of GUS. Subsequently, the characteristics of AMGT1P33 and its involvement in salt tolerance were investigated.

Analysis of the transcription range showed that AMGT1P33 regulates GUS expression in both dicotyledonous (Nicotiana tabacum, Pachyrhizus erosus, and Solanum tuberosum) and monocotyledonous (Agropyron cristatum, Cocos nucifera, and Thalassia hemprichii) plant species. According to quantitative real-time-PCR, the expression level of GUS in N. tabacum when regulated by AMGT1P33 was 5.97 times higher than that when regulated by the 35S promoter. Additionally, the regulation of AmBADH expression by AMGT1P33 in yeast and Arabidopsis significantly improved salt tolerance.

These findings suggest that endogenous salt-tolerance-related promoters play a key role in the salt-tolerance mechanism of A. marina. These findings can be extended to elucidate the salt-tolerance mechanisms in other plants and contribute to the development of new promoter tools and methods for transgenic engineering.