# Deoxynivalenol Determination Using Innovative Lateral Flow Device Technology

## TL;DR

This study evaluates a new digital lateral flow device for detecting deoxynivalenol in wheat, showing strong agreement with a standard lab method.

## Contribution

The study introduces an innovative digital lateral flow device for accurate DON detection in wheat.

## Key findings

- The new device's results closely matched those of the LC-MS/MS method.
- Statistical analysis showed no significant difference between the two methods.
- The correlation between the methods was very strong (r = 0.9968).

## Abstract

Deoxynivalenol (DON), generally the most widespread mycotoxin in wheat, is regulated by the EU regulation in cereals and cereal-derived products. Its presence can be detected by chromatographic or rapid methods; the latter technique is generally used in control analysis, fulfilling the needs of the stakeholders of the wheat grain chain. Lateral flow strips are often used for the rapid detection of different mycotoxins in several agricultural products; regarding DON determination, different lateral flow immunochromatography strips are currently available, also providing quantitative results. The purpose of this work was to evaluate the accuracy of an innovative lateral flow device coupled to a bench top device, following a digital approach. The proposed method was compared to an LC-MS/MS method, analyzing 50 naturally contaminated wheat samples. The results obtained using the two methods were very similar and, applying a paired t-test, the mean difference between measurements resulted not significantly different (α = 0.003). The correlation between the results showed a slope of the line close to 1 (m = 0.9904) and a regression coefficient (r) of 0.9968.